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Occlusion was performed simultaneously with the acquisition of dynamic LV volume from long-axis real-time CMR at 0.55 T, and recordings of invasive LV and aortic pressures, electrocardiogram, and CMR gradient waveforms. PV loops were derived by synchronizing pressure and volume measurements.The optimal method to measure the transaortic gradient in a patient with aortic stenosis is a simultaneous left ventricular (LV) pressure and central aortic (Ao) pressure with side-hole catheters. Shown are examples in which alternative methods are used to obtain the pressures, which produce erroneous results.
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Western blot analysis of vector particles encoding CD19-CAR (CD8-LV, CD4-LV or VSV LV) or GFP (CD8- -LVGFP) as transgene. 3.54x108 particles were separated on a 12% SDS polyacrylamide gel before transferred to a being PVDF membrane. Detection was performed by incubation of the membrane with an antibody specific for the
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